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Table 3 Changes in the canine sperm velocity parameters during cold storage (5 °C) in the extenders containing different levels of melatonin versus the controls

From: Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Sampling Days

Group

Negative Control

Positive Control

M1

M2

M3

ALH1

3.41 ± .20 1

3.28 ± .21 1

3.33 ± .16 1

3.23 ± .25 1

3.34 ± .38 1

ALH3

3.34 ± .23 1

3.26 ± .23 1

3.27 ± .17 1

3.15 ± .26 1

3.36 ± .38 1

ALH5

2.74 ± .31 2

2.82 ± .22 1

2.91 ± .12 1

2.84 ± .11 12

2.86 ± .25 12

ALH7

1.40 ± .14 2a

1.89 ± .32 2ab

1.78 ± .18 2ab

2.12 ± .18 2b

1.72 ± .19 3ab

ALH9

.25 ± .05 3a

.35 ± .05 3a

.47 ± .10 3a

.76 ± .12 3b

.44 ± .07 4a

BCF1

5.04 ± .17 1

4.33 ± .21 1

4.43 ± .20 1

4.60 ± .10 1

4.53 ± .10 1

BCF3

4.99 ± .20 1

4.43 ± .17 1

4.45 ± .20 1

4.78 ± .22 1

4.54 ± .11 1

BCF5

3.91 ± .26 23

4.36 ± .26 12

4.12 ± .21 12

4.33 ± .05 1

4.22 ± .42 1

BCF7

2.22 ± .24 34a

3.10 ± .54 2ab

3.08 ± .36 23ab

3.93 ± .33 1c

2.94 ± .31 2ab

BCF9

.33 ± .05 4a

.44 ± .08 3a

.57 ± .15 4ab

1.00 ± .25 2b

.56 ± .18 3ab

MAD1

66.68 ± 4.62 1

66.18 ± 4.68 1

66.87 ± 2.66 1

64.70 ± 3.77 1

68.97 ± 5.47 1

MAD3

63.39 ± 5.66 1

66.20 ± 4.68 1

66.06 ± 2.74 1

64.70 ± 3.77 1

69.93 ± 5.33 1

MAD5

60.47 ± 6.78 1

60.87 ± 4.32 1

57.18 ± 3.86 23

60.40 ± 2.78 23

57.06 ± 6.01 2

MAD7

27.80 ± 3.39 2a

39.20 ± 7.37 2ab

36.61 ± 2.86 3ab

44.91 ± 4.53 3b

34.25 ± 4.81 3ab

MAD9

3.99 ± .63 3a

6.28 ± 0.76 3ab

8.67 ± 2.26 4ab

11.16 ± 1.91 4b

8.36 ± 1.81 4ab

  1. The different numbers and letters in superscripts indicate a statistically significant difference (P ≤ 0.05) within and between columns, respectively. A two-way repeated-measures ANOVA statistical test was used to make a comparison between treatment groups at different times. Pairwise comparisons with LSD confidence interval adjustments were carried out to examine the simple effects for the time and study groups. Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as Means±SEM. ALH Amplitude of Lateral Head Displacement (μm), BCF Beat Cross Frequency (Hz), and MAD Mean Angular Displacement (°)