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Fig. 12 | BMC Veterinary Research

Fig. 12

From: Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Fig. 12

MTT reduction assay results measured by ELISA UV-VIS microplate reader at a wavelength of 550 nm in the extenders containing different levels of melatonin versus the controls. Absorbance values are differences of ODs at the start and after an incubation period of 1 h at 37 °C. One-way ANOVA and LSD posthoc statistical tests were used to make a comparison between treatment groups. Different letters in bar superscripts indicate a statistically significant difference (P ≤ 0.05). Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). All numbers are presented as mean% ± SD, n = 4

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