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Table 3 Comparison of H5N8 HPAI virus isolation efficiency from tracheal swabs (10/ pool) stored in different transport media

From: Effects of swab pool size and transport medium on the detection and isolation of avian influenza viruses in ostriches

Media

Spiking dose

Virus isolation results (number of passages performed)

Total Positive

Allantoic fluid mean virus titre in EID50 equivalentsb/ ml (mean Ct value)

Replicate 1

Replicate 2

Replicate 3

Replicate 4

Replicate 5

Replicate 6

VTM

High

+ (1)

+ (1)

+ (1)

+ (1)

+ (1)

+ (1)

6/6

106.7 (22.4)

Medium

+ (2)

- (3)

+ (2)

- (3)

+ (3)

- (3)

3/6

104.5 (29.4)

Low

- (3/2)

- (3)

+ (3)

- (3)

- (3/2)

- (3/2)

1/6

103.0 (34.4)

PBS: glycerol

High

+ (1)

+ (1)

- (2)

+ (1)

+ (3)

+ (1)

5/6

106.6 (22.8)

Medium

+ (3/3a)

- (3)

- (3)

- (3)

- (3/2a)

- (3)

1/6

104.4 (30.0)

Low

+ (2)

- (3)

- (3 /2a)

- (3)

- (3)

- (3)

1/6

103.1 (34.1)

  1. Ct Cycle threshold
  2. + Positive
  3. - Negative
  4. aPassage number where the sample was filtered
  5. bEgg Infectious Dose50 equivalents as determined by RT-rtPCR