Table 2 Primers and target genes of Trypanosoma spp. investigated [33,34,35,36]
Primers (5′-3′) | Target gene | Product size (bp) | Cycling conditions (same for all primers) |
|---|---|---|---|
For-Kin: GCGTTCAAAGATTGGGCAAT | ITS1 | T. evansi (540 bp), T. vivax (300 bp) | 94 °C—3 min 94 °C—1 min 58 °C—1 min (× 4) 72 °C—1 min 94 °C—1 min 56 °C—1 min (× 8) 72 °C—1 min 94 °C—1 min 54 °C—1 min (× 23) 72 °C—1 min 72 °C—5 min [37] |
Rev-Kin: CGCCCGAAAGTTCACC | |||
For-ILO: GCCACCACGGCGAAAGAC | Rotat 1.2 VSG | T. evansi (488 bp) | |
Rev-ILO: TAATCAGTGTGGTGTGC | |||
For-IR: GCTGTAGGTGAACTTGCAGCAGCTGGATCATT | ITS | T. evansi (1.1 kbp) | |
Rev-IR: GCGGGTAGTCCTGCCAAACACTCAGGTCTG | |||
For-ITS1: CCGGAAGTTCACCGATATTG | ITS1 | T. evansi (480 bp), T. vivax (250 bp), T. congolense (620 bp). | |
Rev-ITS1: TTGCTGCGTTCTTCAACGAA |