Fig. 2

Agarose gel stained with ethidium bromide (1.5%) showing PCR product of ITS1 using Kin primers (A and B). Gel (A) Lanes 1,2: Trypanosoma evansi (540 bp), Gel (B) Lane 1: T. vivax (300 bp), and Lane 2: Mixed infection of T. evansi and T. vivax. Gel (C) showing PCR product of ITS1 using ITS1 primers; Lanes 1, 2, 3: Mixed infection of Trypanosoma evansi (480 bp), T. vivax (250 bp), and T. congolense (620 bp). Gel (D) showing PCR product of ITS using IR primers; Lanes 1–7: Trypanosoma evansi (1.1 kbp). Gel (E) showing PCR product of Rotat 1.2 VSG region using ILO primers; Lanes 1–6: Trypanosoma evansi (488 bp). Any other lower and higher bands are non-specific. Lane M: Low molecular weight marker (50–1500 bp)