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Table 1 Inter-assay and intra-assay repeatability of the ‘Ca. M. haemosuis’ qPCR

From: Occurrence of ‘Candidatus Mycoplasma haemosuis’ in fattening pigs, sows and piglets in Germany using a novel gap-based quantitative real-time PCR assay

 

inter-assay repeatability (15 runs)

intra- assay repeatability (5 runs)

Copy numbera

mean Ctb (±SD)

mean Ct (±SD)

107

15.27 (± 0.27)

15.31 (± 0.15)

106

17.61 (± 0.82)

17.67 (± 0.13)

105

21.08 (± 0.60)

21.33 (± 0.10)

104

24.62 (± 0.64)

24.80 (± 0.10)

103

27.68 (± 0.82)

27.69 (± 0.09)

102

30.79 (± 0.70)

30.84 (± 0.15)

101

33.51 (± 0.75)

33.52 (± 0.14)

  1. aCopy numbers (GE/reaction) of the plasmid pC_CMhsuis were calculated from spectrophotometrically quantified DNA as described in Methods
  2. bMean threshold cycle values and standard deviations