Development of a one-step multiplex qRT–PCR assay for the detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus

Table 1 Comparison of the Ct values between the singleplex and multiplex qRT-PCR

Plasmid	Concentration (copies/μL)	2.52 × 10⁷	2.52 × 10⁶	2.52 × 10⁵	2.52 × 10⁴	2.52 × 10³	2.52 × 10²	2.52 × 10¹	2.52 × 10⁰	2.52 × 10⁻¹
p-ASFV	Singleplex qRT-PCR	13.781	17.478	20.347	24.044	28.274	31.010	35.073	39.481	–
p-ASFV	Multiplex qRT-PCR	13.846	16.859	20.052	24.052	27.862	31.227	34.776	39.351	–
p-CSFV	Singleplex qRT-PCR	13.490	17.195	20.329	24.584	27.974	31.427	34.793	39.341	–
p-CSFV	Multiplex qRT-PCR	13.788	17.011	20.433	24.418	28.270	31.837	35.074	39.857	–
p-APPV	Singleplex qRT-PCR	12.172	15.430	19.161	23.145	27.183	30.502	33.480	36.519	–
p-APPV	Multiplex qRT-PCR	11.976	15.469	18.845	22.955	26.767	30.154	34.328	36.830	–

ISSN: 1746-6148