Table 2 Primers and TaqMan probes used for detection of C. abortus and internal amplification control by real-time PCR
Name | Sequence | Amplicon size (bp) |
|---|---|---|
CpaOMP1-F CpaOMP1-R CpaOMP1-Sa | 5-GCAACTGACACTAAGTCGGCTACA-3 5-ACAAGCATGTTCAATCGATAAGAGA-3 FAMb- TAAATACCACGAATGGCAAGT TGGTTTAGCG-TAMRAc | 82 |
EGFP-1-F EGFP-10-R EGFP-HEXd | 5-GACCACTACCAGCAGAACAC-3 5-CTTGTACAGCTCGTCCATGC-3 HEXd- AGCACCCAGTCCGCCCTGAGCABHQ1 e | 177 |