Table 1 The sequence of primers of Chlamydiales and C. abortus used in the PCR assay

Borel et al, 2006 [21]

352 bp

Forward 5′-CAA GGT GAG GCT GAT GAC-3′

Reverse 5′-TCG CCT KTC AAT GCC AAG-3’

Chlamydiales

16 s–23 s spacer region

(100pmoles/ ul)

Longbottom et al 2002 [22]

222 bp

Forward 5’- TTTTCAGGATCCTATTGTCCTCCAGGCA −3′

Reverse 5′- GTGTCGACATCAGCATAAATAGCCCCG−3′

Chlamydia. abortus

pmp1 gene

(100 pmoles/ ul)