Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle

Table 2 Effects of transport and rumen-protected creatine pyruvate on the contents of immunoglobulin and inflammatory factor in serum, lipopolysaccharide and extracellular DNA in serum and rumen fluid of beef cattle

Item	Treatment¹				SEM	P-value
	Transport		Nontransport			P-value
	RP-CrPyr₁₄₀	RP-CrPyr₀	RP-CrPyr₁₄₀	RP-CrPyr₀		Transport	RP-CrPyr	Transport × RP-CrPyr²
Serum
IgG (g/L)	9.44	8.75	9.93	9.80	0.607	0.539	0.744	0.820
IgM (g/L)	2.44	2.36	2.59	2.47	0.167	0.689	0.776	0.960
IgA (g/L)	0.34	0.36	0.44	0.47	0.021	0.028	0.543	0.983
TNF-α (pg/mL)	59.24	47.91	46.75	57.89	2.594	0.791	0.984	0.040
IFN-γ (pg/mL)	31.92	33.45	41.83	47.25	1.573	0.005	0.301	0.554
IL-1β (pg/mL)	22.87	18.86	28.83	30.89	1.643	0.026	0.773	0.383
IL-6 (pg/mL)	87.00^c	113.72^bc	149.19^a	128.75^ab	4.301	0.002	0.725	0.025
LPS (EU/mL)	16.71^b	29.18^a	11.00^b	10.96^b	2.463	0.001	0.032	0.031
eDNA (ng/μL)	2.36	2.39	2.11	2.17	0.146	0.459	0.882	0.975
Rumen
LPS (EU/mL)	2.19	2.41	0.64	0.89	0.074	< 0.001	0.143	0.929
eDNA (ng/μL)	19.95	20.94	26.12	17.00	1.503	0.720	0.213	0.131

^a,b,c Means within a row with no common superscript differ significantly (P < 0.05)
¹ A 2 × 2 factorial arrangement with 2 supplemental levels of rumen-protected creatine pyruvate (RP-CrPyr) in basal diets (0 or 140 g/d for each cattle) and 2 transport treatment (transport 0 or 12 h before slaughter)
² Transport × RP-CrPyr, the interaction between Transport and RP-CrPyr
SEM Stand error of mean, TNF-α Tumor necrosis factor-α, IFN-γ Interferon γ, IL-1β Interleukin-1β, IL-6 Interleukin-6, LPS Lipopolysaccharide, eDNA Extracellular DNA

ISSN: 1746-6148