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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells

Fig. 5

Post-storage differentiation ability of cADMSCs. cADMSCs were suspended in various carrier solutions at 4 °C for different time points. GAPDH was used as the housekeeping control gene. All mRNA data were normalized to the level of GAPDH and relative fold changes in expression level were measured. (A) The expression of COL2A was significantly higher in saline until 6 h and in Hepa-Sal at 12 h. PBS showed the lowest expression at 12 h of storage. (B) The expression of Sox9 of cADMSCs stored in HS was significantly higher at 1 and 6 h of storage. cADMSCs stored in Hepa-Sal at 12 h showed the highest expression of Sox9. Differentiated cells not stored in carrier solutions served as positive controls. The results are representative of three independent experiments. The bar graph represents the mean ± SD. cADMSCs - canine adipose-derived mesenchymal stem cells, GAPDH - Glyceraldehyde-3-Phosphate Dehydrogenase, COL2A - collagen type IIa, Saline - 0.9% saline, PBS - Phosphate-buffered saline, 5% DS - 5% Dextrose solution, Hepa-Sal - Heparin in saline, HS - Hartmann’s solution, NC - Negative control, SD - Standard deviation. (*P < 0.05), (**P < 0.001), (***P < 0.0001)

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