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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells

Fig. 2

Post-storage proliferation and viability of cADMSCs. cADMSCs were suspended in different carrier solutions in a time-dependent manner at 4 °C. A Proliferation was determined by the cell counting kit-8 assay. cADMSCs showed no significant decrease in proliferation until 6 h of storage. However, there was significantly higher proliferation at 12 h of storage in 5% DS. B Viability was measured by the MTT assay. The viability of cADMSCs decreased significantly over time. Cells not stored in carrier solutions served as controls. The results are representative of three independent experiments. The bar graph represents the mean ± SD. cADMSCs - canine adipose-derived mesenchymal stem cells, Saline - 0.9% saline, MTT - 3,4,5 – dimethylthiazol-2-yl)-2–5-diphenyltetrazolium bromide, PBS - Phosphate-buffered saline, 5% DS - 5% Dextrose solution, Hepa-Sal - Heparin in saline, HS - Hartmann’s solution, SD - Standard deviation. (*P < 0.05, **P < 0.001, and ***P < 0.0001)

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