Molecular characterization and phylogenetic analysis of bovine ephemeral fever viruses in Khuzestan province of Iran in 2018 and 2020

Table 2 List of primes used for detection and sequencing of glycoprotein (G) gene

Type		Primer Name	Sequence 5′-3′	Reference
Nested-PCR for Detection	First Run	G1F	ATGTTCAAGGTCCTCATAATTACC	32¹
	First Run	G4R	AATGATCAAAGAACCTATCATCAC
	Second Run	420F	AGAGCTTGGTGTGAATAC	31
	Second Run	420R	CCAACCTACAACAGCAGATA
Nested-PCR for sequencing	First Run	G1F	ATGTTCAAGGTCCTCATAATTACC	32¹
	First Run	G4R	AATGATCAAAGAACCTATCATCAC
	Second Run	G1F	ATGTTCAAGGTCCTCATAATTACC	32
		G1R	GCTTGTGTTGTATTAGGA
		G2F	GGAATACGGAGATGAATCAA	32
		G2R	ATTCTGTTCTATCTGTGTGC
		G3F	TTGAGGATGGAGAATGGTGG	32
		G3R	TACAACAGCAGATAAAAC
		G4F	AAATGGAATGATCTTTGTGG	32
		G4R	AATGATCAAAGAACCTATCATCAC

¹G4R primer was modified based on the Middle East strains of India 2019 (MN905763) and Israel 2000 (JN833630)

ISSN: 1746-6148