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Table 1 Sequences of oligonucleotides used for PCR in this study

From: The recombinant pseudorabies virus expressing porcine deltacoronavirus spike protein is safe and effective for mice

Name

Sequence (5′-3′)

Purpose

gE

ATCTGGACGTTCCTGCCC

Detecting PRV gE gene

Amplifying the TK gene of PRV

GTAGATGCAGGGCTCGTACA

TK

GATGACATACACATGGCTTTATACGCGCC

TCACCGCCGCGGCCCGGCGACGTACTC

gI

TCGCCGAGCAACTACAGCGG

CGGCGTCGTCGTCTCCGCGT

Detecting PRV gI gene

Amplifying the S gene of PDCoV

S

CCAGCAAGTTGACCGTCTCA

CCATTTGGTGCAGTCTGTGT

pMD-S

TTGTTAACCAGCAGGGCGAG

AGCCAACCGTCCTGTGATG

Detection PDCoV S gene for qPCR

pEGFP-gI28k-PDCoV-S

TCGAGCTCAAGCTTCGAATTCCAGCAAACTTCTGAGGCTCTT

Construction of recombinant transfer plasmids

CGAGCCGGGGGAGATACGCGTATACCACGGCCGTTTAAGGTAAGT