Table 2 Oligonucleotide primers used in qRT-PCR analysisa
Gene name | Sequence (forward, reverse) | Reference |
|---|---|---|
Housekeeping gene | ||
 GAPDH | F: 5′- GAGGTCGGAGTGAACGGAT −3’ | [16] |
R: 5′- CCTGGGTCGAATCATACTGGAACA − 3′ | ||
Inflammatory cytokines | ||
 IL-8 | F: 5′- TTTCTGCAGCTCTCTGTGAGG −3’ | [17] |
R: 5′- CTGCTGTTGTTGTTGCTTCTC − 3′ | ||
 IFN-γ | F: 5′- GTTTTTCTGGCTCTTACTGC − 3’ | [18] |
R: 5′- CTTCCGCTTTCTTAGGTTAG − 3′ | ||
 TNF-α | F: 5′- ATCGGCCCCCAGAAGGAAGAG − 3’ | [19] |
R: 5′- GATGGCAGAGAGGAGGTTGAC − 3′ | ||
 IL-10 | F: 5′- GCATCCACTTCCCAACCA − 3’ | [20] |
R: 5′- CTTCCTCATCTTCATCGTCAT − 3′ | ||
Tight junction proteins | ||
 ZO-1 | F: 5′- AAGCCCTAAGTTCAATCACAATCT − 3’ | [21] |
R: 5′- ATCAAACTCAGGAGGCGGC − 3′ | ||
 OCLD | F: 5′- TCCTGGGTGTGATGGTGTTC − 3’ | [21] |
R: 5′- CGTAGAGTCCAGTCACCGCA − 3′ | ||
 CLDN1 | F: 5′- TCGACTCCTTGCTGAATCTG − 3’ | [16] |
R: 5′- TTACCATACCTTGCTGTGGC − 3′ | ||
Mucin production | ||
 MUC2 | F: 5′- GGCTGCTCATTGAGAGGAGT − 3’ | [22] |
R: 5′- ATGTTCCCGAACTCCAAGG − 3’ | ||