Fig. 6

Alizarin red staining of canine MSCs in osteogenic medium after 21 days. (A) Control and osteogenic cells show deposition of hydroxyapatite mineral stained red with Alizarin red stain (objective 20×). Scale Bar = 20 μm. (B) The ALP activity of differentiated osteocytes compared to undifferentiated control cells. The data was normalized by unit per gram of protein and expressed as mean ± SEM. ^A-CDifferent superscripts letters indicate significant differences (P < 0.05). (C) Immuno-expression of osteopontin in canine MSCs recovered from all four fat sources and kept in osteogenic media showed a positive expression of Osteopontin. Scale bar = 25 μm. Abbreviations: S/C: Subcutaneous, PR: Perirenal, OM: Omentum, IPFP: Infrapatellar fat pad