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Fig. 7 | BMC Veterinary Research

Fig. 7

From: Investigation of the effects of mTOR inhibitors rapamycin and everolimus in combination with carboplatin on canine malignant melanoma cells

Fig. 7

Immunoblotting of phosphorylated and native upstream and downstream proteins of mTOR in the PI3K/AKT/mTOR pathway. Analysis performed after 24-h treatment with carboplatin (15 μM), everolimus (10 nM), or rapamycin (10 nM) as single agents or in combination. The top table indicates the use of vehicle (−) or drug (+). Vehicles were DMSO for mTOR inhibitors and growth medium for carboplatin. Protein target indicated on the left with corresponding molecular weight on the right. AKT, protein kinase B; DMSO, dimethyl sulfoxide; kDa, kilodalton; mTOR, mechanistic target of rapamycin; p-AKT, phosphorylated protein kinase B; p-mTOR, phosphorylated mechanistic target of rapamycin; p-p70S6K, phosphorylated p70-s6 kinase; p70S6K, p70-s6 kinase

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