Fig. 7From: Investigation of the effects of mTOR inhibitors rapamycin and everolimus in combination with carboplatin on canine malignant melanoma cellsImmunoblotting of phosphorylated and native upstream and downstream proteins of mTOR in the PI3K/AKT/mTOR pathway. Analysis performed after 24-h treatment with carboplatin (15 μM), everolimus (10 nM), or rapamycin (10 nM) as single agents or in combination. The top table indicates the use of vehicle (−) or drug (+). Vehicles were DMSO for mTOR inhibitors and growth medium for carboplatin. Protein target indicated on the left with corresponding molecular weight on the right. AKT, protein kinase B; DMSO, dimethyl sulfoxide; kDa, kilodalton; mTOR, mechanistic target of rapamycin; p-AKT, phosphorylated protein kinase B; p-mTOR, phosphorylated mechanistic target of rapamycin; p-p70S6K, phosphorylated p70-s6 kinase; p70S6K, p70-s6 kinaseBack to article page