Fig. 6

Apoptosis after treatment with carboplatin, everolimus or rapamycin. Analysis performed after 24-h and 72-h of treatment with carboplatin (15 μM), everolimus (10 nM), or rapamycin (10 nM) as a single agent or in combination. Combined analysis of all cell lines for early and late apoptosis fractions comparing single agent, combination, and vehicle treatments. Cells were stained with FITC-conjugated annexin V and PI and staining was evaluated by flow cytometry. X-axis and Y-axis represent treatments administered and % apoptosis, respectively. The error bars represent mean +/− SD of 3 experiments conducted in technical duplicate. Significance was recorded at ** p < .01, *** p < .001, **** p < .0001 using a one-way ANOVA, Dunnett’s multiple comparisons test, comparing treated groups to the vehicle control (DMSO). C, carboplatin; DMSO, dimethyl sulfoxide; E, everolimus; PI, propidium iodide; n.s., non-significance; R, rapamycin