Table 3 The primers used in the study
Primers | Sequence (5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
CIAV-F | CAGAATTCCCACCTCAAGCGACTTCGAC | 580 | 54 |
CIAV-R | ATGTCGACGGGGCTGAAGGAT | ||
REV-F | CATACTGAGCCAATGGTT | 300 | 54 |
REV-R | AATGTTGTAGCGAAGTACT | ||
MDV-F | TCATCAGGGTCTCCCGTCACCT | 1005 | 55 |
MDV-R | AGAGATGTCTCAGGAGCCAGAG | ||
FAdV-F | AATTTCGACCCCATGACGCGCCAGG | 508 | 56 |
FAdV-R | TGGCGAAAGGCGTACGGAAGTAAGC | ||
ALV-J-F | GGATGAGGTGACTAAGA | 512 | 56 |
ALV-J-R | CGAACCAAAGGTAACACACG | ||
Avian HEV-F1 | TCGCCT(C)GGTAAT(C)ACA(T)AATGC | 278 | 60 |
Avian HEV-R1 | GCGTTC(G)CCG(C)ACAGGT(C)CGGCC | ||
Avian HEV-F2 | ACA(T)AATGCT(C)AGGGTCACCCG | 242 | 56 |
Avian HEV-R2 | ATGTACTGA(G)CCA(G)CTG(C)GCCGC |