Prevalence, co-infection and seasonality of fecal enteropathogens from diarrheic cats in the Republic of Korea (2016–2019): a retrospective study

Table 1 The details of real-time PCR for the detection of feline enteropathogens

Pathogen	Target gene	Real-time PCR conditions
Pathogen	Target gene	PCR protocol	Primer/Probe concentration	LOD^c (Based on Ct 40)
Feline coronavirus	M	RT PCR^a	Primer: 10pmole/Rx Probe: 5pmole/Rx	10 copies
C. perfringens	cpa	PCR^b	//	100 copies/Rx
C. coli	gyrB	PCR	//	100 copies/Rx
Feline parvovirus	vp2	PCR	//	10 copies/Rx
T. foetus	ITS1	PCR	//	100 copies/Rx
EPEC	bfpA	PCR	//	100 copies/Rx
Giardia lamblia	18 s rRNA	PCR	//	10 copies/Rx
ETEC	ST/LT	PCR	//	100 copies/Rx
C. jejuni	rimB	PCR	//	100 copies/Rx
EHEC	stx1/stx2	PCR	//	10 copies/Rx
Group A Rotavirus	nsp4	RT PCR	//	10 copies/Rx
C. parvum	18 s rRNA	PCR	//	100 copies/Rx
C. cayetanensis	18 s rRNA	PCR	//	100 copies/Rx
EIEC	ipaH	PCR	//	100 copies/Rx
Salmonella spp.	invE	PCR	//	100 copies/Rx
E. histolytica	18 s rRNA	PCR	//	100 copies/Rx
Toxocara cati	ITS1	PCR	//	100 copies/Rx
Toxoplasma gondii	RE	PCR	//	100 copies/Rx
FIV	gag	PCR	//	100 copies/Rx

C. perfringens Clostridium perfringens, C. coli Campylobacter coli, T. foetus Tritrichomonas foetus, EPEC enteropathogenic Escherichia coli, ETEC enterotoxigenic Escherichia coli, C. jejuni Campylobacter jejuni, EHEC enterohemorrhagic Escherichia coli, C. parvum Cryptosporidium parvum, C. cayetanensis Cyclospora cayartensis, EIEC enteroinvasive Escherichia coli, E. histolytica Entamoeba histolytica, FIV feline immunodeficiency virus
^aPCR thermal condition: 95 °C, 5 min (95 °C, 10 s – 60 °C, 30 s; 45 cycles)
^bRT PCR thermal condition: 50 °C, 15 min – 95 °C, 5 min (95 °C, 10 s – 60 °C, 30 s; 45 cycles)
^cLOD (limitation of detection) was determined to be 10 folds serial dilutions of synthetic plasmid including target gene of individual pathogen based on a C_T value of 40

ISSN: 1746-6148