Fig. 2
From: Characterization and reverse genetic establishment of cattle derived Akabane virus in China
Simplified scheme for the construction of TVT7R series. The upper part of the figures shows the sequence around BbsI restriction sites of TVT7R that is used to insert AKAV S, M or L segment. RNA transcripts produced by bacteriophage T7 RNA polymerase would contain one G residues, derived from the cloning site, before the authentic AKAV 5′ terminal sequence. The exact 3′ end of the transcript RNA was specified self-cleavage by the hepatitis delta virus (HDV) antigenome ribozyme. The conserved terminal bases of all three AKAV segments are shown. T7, T7 promoter; T7 term, T7 transcription termination sequence