Fig. 5
From: Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
Porcine cornea preparation procedure: porcine eyes showing the filter paper placed on the center of the ocular surface (a); application of a plastic ring on the L cornea (b); application of isoosmolar 0.1% riboflavin drops into the plastic ring for 30 min (c); details of UV-A lamp during riboflavin/UV-A corneal phototherapy (d). To obtain the cultured condition, the corneas were isolated from the bulbs and preserved in a specific culture medium for corneal deturgescence (Carry-C® medium, Alchimia, Padua, Italy) for 7 days in an incubator at 37 °C with 5% CO2 [24]