Table 3 Details of the RT-qPCR assays used to validate the RNA-Seq results
Accession numbera | Gene symbol | Gene name | Sequence (5′➔ 3′) |
|---|---|---|---|
XM_004018378 | IL17RB | Interleukin17 receptor B | F:TTCTCAACTACCACACTA R:AAGGATAACTTCACTTCTG |
XM_012097111.2 | IL1RAP | interleukin 1 receptor accessory protein | F:CCAAGTGTATGAAGATGAA R:TACCAGATGAGAGTAAGG |
XM_015101624.1 | P2RX7 | purinergic receptor P2X7 | F:AGAGTCAAGAGATTCAGT R:GTAGGTTGGCTAATAAGG |
XM_004018807.3 | TREM2 | triggering receptor expressed on myeloid cells 2 | F:AATACCGATGAGGAGAAG R:CTGTGGAAATGGAAGAAC |
NM_001198545.1 | SBD2 | beta defensin 2 | F:TGGGTCAGGATTTACTCAT R:CTTGGTCAGCACACAGAT |
NM_001278560.1 | PTGER2 | prostaglandin E receptor 2 | F:GTGTCTTGTGATTGCTTAT R:CCAACTGAACCTACTCTT |
NM_001301405.2 | BPIFA1 | BPI fold containing family A member 1 | F:TCTCTGCTTGATGGATTG R:CTCAGGAAGGACATTATTCA |
NM_001252183.1 | GATA3 | GATA binding protein 3 | F:CGTGGTGTCTGTGTTCTC R:ATAGGGAATGTGAGTCTGAATG |
XM_012179609.2 | NFKB1 | nuclear factor kappa B subunit 1 | F:CTAAGATATTCCGAGCAGAA R:CTAACCTACAGTGTCCTATG |
XM_004012818.3 | TBX21 | T-box 21 | F:CAGAACGCCGAGATTACT R:ACGGATACATACATGGATTCA |
NM_001190390.1 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | F:TGCCAAGTATGATGAGAT R:TCAGTGTAGCCTAGAATG |