Whole-genome sequencing reveals high genetic diversity of Streptococcus uberis isolated from cows with mastitis

Silva, Nathália Cristina Cirone; Yang, Yongqiang; Rodrigues, Marjory Xavier; Tomazi, Tiago; Bicalho, Rodrigo Carvalho

doi:10.1186/s12917-021-03031-4

BMC Veterinary Research

Table 2 Distribution of genes associated with antimicrobial resistance of 159 Streptococcus uberis isolated from clinical mastitis according to clinical outcomes recorded during the follow-up period

From: Whole-genome sequencing reveals high genetic diversity of Streptococcus uberis isolated from cows with mastitis

Resistance factor	Product	Antibiotic class	Mortality (% of cows infected by positive strains)				Bacteriological cure				Clinical cure				Loss of mammary quarter function.				Reoccurrence of CM				Total of positive strains
			Dead		Alive		No		Yes		No		Yes		No		Yes		No		Yes
			n	%	n	%	n	%	n	%	n	%	n	%	n	%	n	%	n	%	n	%
rlmA(II)	23 S rRNA (guanine(748)-N(1))-methyltransferase	Macrolides, Lincosamides	31	19	28	81	88	55	71	45	20	13	139	87	142	89	17	11	121	76	38	24	159
rpoB	DNA-directed RNA polymerase beta subunit	Rifamycins, Peptide antibiotics	31	19	128	81	88	55	71	45	20	13	139	87	142	89	17	11	121	76	38	24	159
rpoC	DNA-directed RNA polymerase beta’ subunit	Myxopyronins Corallopyronins, Peptide antibiotics	31	19	128	81	88	55	71	45	20	13	139	87	142	89	17	11	121	76	38	24	159
S10p	SSU ribosomal protein S10p	Tetracyclines, Glycylcyclines	30	19	128	81	87	55	71	45	20	13	138	87	141	89	17	11	120	76	38	24	158
alr	Alanine racemase	Cycloserine	31	20	126	80	86	55	71	45	20	13	137	87	140	89	17	11	119	76	38	24	157
EF-G	Translation elongation factor G	Fusidic acid	31	20	126	80	87	55	70	45	20	13	137	87	140	89	17	11	119	76	38	24	157
S12p	SSU ribosomal protein S12p	Aminoglycosides	31	20	126	80	87	55	70	45	20	13	137	87	140	89	17	11	119	76	38	24	157
pgsA	CDP-diacylglycerol–glycerol-3-phosphate 3-phosphatidyltransferase	Peptide antibiotics (daptomycin)	28	18	127	82	85	55	70	45	20	13	135	87	138	89	17	11	121	78	34	22	155
fabK	Enoyl-[acyl-carrier-protein] reductase [FMN, NADH]	Triclosan	30	19	124	81	84	55	70	45	20	13	134	87	138	90	16	10	116	75	38	25	154
kasA	3-oxoacyl-[acyl-carrier-protein] synthase, KASII	Isoniazid, Triclosan	30	19	124	81	84	55	70	45	20	13	134	87	138	90	16	10	116	75	38	25	154
D	Lincosamide nucleotidyltransferase = > Lnu(D)	Lincosamides	30	20	122	80	83	55	69	45	20	13	132	87	135	89	17	11	114	75	38	25	152
liaF	Membrane protein LiaF(VraT), specific inhibitor of LiaRS(VraRS) signaling pathway	Peptide antibiotics (daptomycin)	30	20	121	80	84	56	67	44	20	13	131	87	134	89	17	11	113	75	38	25	151
LiaR	Cell envelope stress response system LiaFSR, response regulator LiaR(VraR)	Peptide antibiotics (daptomycin)	30	20	121	80	84	56	67	44	20	13	131	87	134	89	17	11	113	75	38	25	151
LiaS	Cell envelope stress response system LiaFSR, sensor histidine kinase LiaS(VraS)	Peptide antibiotics (daptomycin)	30	20	121	80	84	56	67	44	20	13	131	87	134	89	17	11	113	75	38	25	151
murA	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	Fosfomycin	30	20	121	80	84	56	67	44	20	13	131	87	134	89	17	11	113	75	38	25	151
gidB	16 S rRNA (guanine(527)-N(7))-methyltransferase	Aminoglycosides	29	19	121	81	83	55	67	45	20	13	130	87	133	89	17	11	112	75	38	25	150
GdpD	Glycerophosphoryl diester phosphodiesterase	Peptide antibiotics (daptomycin)	30	20	119	80	83	56	66	44	20	13	129	87	132	89	17	11	111	74	38	26	149
folP	Dihydropteroate synthase	Sulfonamides	30	20	117	80	84	57	63	43	20	14	127	86	131	89	16	11	109	74	38	26	147
ddl	D-alanine–D-alanine ligase	Cycloserine	29	20	117	80	81	55	65	45	20	14	126	86	129	88	17	12	108	74	38	26	146
Iso-tRNA	Isoleucyl-tRNA synthetase	Mupirocin	30	21	116	79	83	57	63	43	20	14	126	86	130	89	16	11	109	75	37	25	146
folA, Dfr	Dihydrofolate reductase	Diaminopyrimidines	29	20	115	80	81	56	63	44	20	14	124	86	128	89	16	11	107	74	37	26	144
gyrB	DNA gyrase subunit B	Novobiocin	29	20	115	80	80	56	64	44	20	14	124	86	127	88	17	12	106	74	38	26	144
gyrA	DNA gyrase subunit A	Ciprofloxacin	30	21	113	79	80	56	63	44	20	14	123	86	127	89	16	11	106	74	37	26	143
EF-Tu	Translation elongation factor Tu	Elfamycins	29	21	112	79	77	55	64	45	20	14	121	86	127	90	14	10	105	74	36	26	141
tetM	Tetracycline resistance, ribosomal protection type = > Tet(M)	Tetracyclines	6	13	42	88	20	42	28	58	6	13	42	88	43	90	5	10	39	81	9	19	48
parC	DNA topoisomerase IV subunit A	Ciprofloxacin	0	00	7	100	4	57	3	43	2	29	5	71	6	86	1	14	7	100	0	00	7
ant (6)-I	Aminoglycoside 6-nucleotidyltransferase	Aminoglycosides	1	25	3	75	1	25	3	75	0	00	4	100	4	100	0	00	4	100	0	00	4
bceR	Two-component response regulator BceR	Peptide antibiotics (bacitracin)	1	50	1	50	1	50	1	50	0	00	2	100	2	100	0	00	1	50	1	50	2
lsa(E)	ABC-F type ribosomal protection protein	Lincosamides	0	00	1	100	0	00	1	100	0	00	1	100	1	100	0	00	1	100	0	00	1

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ISSN: 1746-6148

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