Phosphate donor | Relative activity |
---|
ATP | 100 |
GTP | 16.4 |
CTP | 13.1 |
UTP | 18.9 |
dATP | 97.1 |
dGTP | 10.8 |
dCTP | 12.6 |
dTTP | < 0.01 |
- The assays were performed by using a radiochemical assay with [3H]-dThd as the substrate at 37 °C. The concentration of dThd was 0.1 mM and the phosphate donor was 1 mM. The data are given as percentage of that with ATP as the phosphate donor (2.56 μmol/mg/min)