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Table 2 Phosphate donor specificity

From: Feline thymidine kinase 1: molecular characterization and evaluation of its serum form as a diagnostic biomarker

Phosphate donor

Relative activity

ATP

100

GTP

16.4

CTP

13.1

UTP

18.9

dATP

97.1

dGTP

10.8

dCTP

12.6

dTTP

< 0.01

  1. The assays were performed by using a radiochemical assay with [3H]-dThd as the substrate at 37 °C. The concentration of dThd was 0.1 mM and the phosphate donor was 1 mM. The data are given as percentage of that with ATP as the phosphate donor (2.56 μmol/mg/min)