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Table 3 List of reference genes and target genes used for RT-qPCR

From: Characterization of receptor tyrosine kinase activation and biological activity of toceranib phosphate in canine urothelial carcinoma cell lines

Gene/pimers Primer sequences Amplicon size (bp) Primer efficiency Correlation coefficient (R2)
Canine PDGFRA, NCBI Reference Sequence: XM_038685627.1
 Canine PDGFRA 1917F 5’-GCTCTCATGTCGGAACTGAAG-3’ 382 96.8% 0.93
 Canine PDGFRA 2152R 5’-GTGTGCTGTCATCAGCAGG-3’
Canine PDGFRB, NCBI Reference Sequence: NM_001003382.1
 Canine PDGFRB 2286F 5’-GACGAGTCAGTGGATTACGTG-3’ 327 97.1% 0.93
 Canine PDGFRB 2612R 5’-GTCTCTCATGATGTCACGAGCCAG-3’
Canine KIT, NCBI Reference Sequence: NM_001003181.1
 Canine KIT 260F 5’-GAGAACACACACAACGAATG-3’ 183 97.6% 0.97
 Canine KIT 442R 5’-GCAGCGGACCAGCGTATCATTG-3’
Canine KDR, NCBI Reference Sequence: NM_001048024.1
 Canine VEGFR2 1537F 5’-GTAAGTACCCTTGTTATCCAAGCAGCC-3’ 192 98.3% 0.96
 Canine VEGFR2 1728R 5’-CGTAGTTCTGTCTGCAGTGCACCAC-3’
Canine GAPDH, NCBI Reference Sequence: NM_001003142.2
 Canine GAPDH 588F 5’-GTCCATGCCATCACTGCCACCCAG-3’ 193 97.4 0.96
 Canine GAPDH 780R 5’-CTGATACATTGGGGGTGGGGACAC-3’
  1. The table shows a list of primers utilized in performing real time quantitative PCR. Listed are sequences for each primer/ gene as well as their size, R2, and listed primer efficiency by the supplier