Characterization of receptor tyrosine kinase activation and biological activity of toceranib phosphate in canine urothelial carcinoma cell lines

Table 3 List of reference genes and target genes used for RT-qPCR

Gene/pimers	Primer sequences	Amplicon size (bp)	Primer efficiency	Correlation coefficient (R²)
Canine PDGFRA, NCBI Reference Sequence: XM_038685627.1
Canine PDGFRA 1917F	5’-GCTCTCATGTCGGAACTGAAG-3’	382	96.8%	0.93
Canine PDGFRA 2152R	5’-GTGTGCTGTCATCAGCAGG-3’	382	96.8%	0.93
Canine PDGFRB, NCBI Reference Sequence: NM_001003382.1
Canine PDGFRB 2286F	5’-GACGAGTCAGTGGATTACGTG-3’	327	97.1%	0.93
Canine PDGFRB 2612R	5’-GTCTCTCATGATGTCACGAGCCAG-3’	327	97.1%	0.93
Canine KIT, NCBI Reference Sequence: NM_001003181.1
Canine KIT 260F	5’-GAGAACACACACAACGAATG-3’	183	97.6%	0.97
Canine KIT 442R	5’-GCAGCGGACCAGCGTATCATTG-3’	183	97.6%	0.97
Canine KDR, NCBI Reference Sequence: NM_001048024.1
Canine VEGFR2 1537F	5’-GTAAGTACCCTTGTTATCCAAGCAGCC-3’	192	98.3%	0.96
Canine VEGFR2 1728R	5’-CGTAGTTCTGTCTGCAGTGCACCAC-3’	192	98.3%	0.96
Canine GAPDH, NCBI Reference Sequence: NM_001003142.2
Canine GAPDH 588F	5’-GTCCATGCCATCACTGCCACCCAG-3’	193	97.4	0.96
Canine GAPDH 780R	5’-CTGATACATTGGGGGTGGGGACAC-3’	193	97.4	0.96

The table shows a list of primers utilized in performing real time quantitative PCR. Listed are sequences for each primer/ gene as well as their size, R², and listed primer efficiency by the supplier

ISSN: 1746-6148