Table 3 List of reference genes and target genes used for RT-qPCR
Gene/pimers | Primer sequences | Amplicon size (bp) | Primer efficiency | Correlation coefficient (R2) |
|---|---|---|---|---|
Canine PDGFRA, NCBI Reference Sequence: XM_038685627.1 | ||||
Canine PDGFRA 1917F | 5’-GCTCTCATGTCGGAACTGAAG-3’ | 382 | 96.8% | 0.93 |
Canine PDGFRA 2152R | 5’-GTGTGCTGTCATCAGCAGG-3’ | |||
Canine PDGFRB, NCBI Reference Sequence: NM_001003382.1 | ||||
Canine PDGFRB 2286F | 5’-GACGAGTCAGTGGATTACGTG-3’ | 327 | 97.1% | 0.93 |
Canine PDGFRB 2612R | 5’-GTCTCTCATGATGTCACGAGCCAG-3’ | |||
Canine KIT, NCBI Reference Sequence: NM_001003181.1 | ||||
Canine KIT 260F | 5’-GAGAACACACACAACGAATG-3’ | 183 | 97.6% | 0.97 |
Canine KIT 442R | 5’-GCAGCGGACCAGCGTATCATTG-3’ | |||
Canine KDR, NCBI Reference Sequence: NM_001048024.1 | ||||
Canine VEGFR2 1537F | 5’-GTAAGTACCCTTGTTATCCAAGCAGCC-3’ | 192 | 98.3% | 0.96 |
Canine VEGFR2 1728R | 5’-CGTAGTTCTGTCTGCAGTGCACCAC-3’ | |||
Canine GAPDH, NCBI Reference Sequence: NM_001003142.2 | ||||
Canine GAPDH 588F | 5’-GTCCATGCCATCACTGCCACCCAG-3’ | 193 | 97.4 | 0.96 |
Canine GAPDH 780R | 5’-CTGATACATTGGGGGTGGGGACAC-3’ | |||