Fig. 4

Curcumol (0.025, 0.0125 and 0.00625 mg/mL) and ribavirin (0.25, 0.125 and 0.0625 mg/mL) were selected to treat EMCV-infected HEK-293 T cells for 24 h, and the expression of MDA5, MAVS, TANK, IRF3, P-IRF3 protein were detected by Western blot. Densitometric values of protein bands were quantified by the Image J. Data were analyzed using GraphPad Prism™ software 5.0 (GraphPad Software, Inc. California, USA). One-way analysis of variance (ANOVA) followed by a Dunnett’s post-test was used to determine the difference between the groups. All groups are compared with EMCV-infected group (* P < 0.05, ** P < 0.01, *** P < 0.001)