Fig. 1
From: Brucella melitensis UGPase inhibits the activation of NF-κB by modulating the ubiquitination of NEMO
Overview of treatments and proteome profiling. (A) Schematic illustration of the experimental procedures used in the present study. Briefly, RAW264.7 cells were subjected to B. melitensis 16 M or 16 M-UGPase− infection for 11 h at an MOI of 100. Then, total protein was extracted from the infected cells and digested by trypsin. The tryptic peptides were desalted and enriched by immunoaffinity purification and then analyzed using LC-MS/MS. (B) Repetitive analysis. The heat map was generated by calculating Pearson’s correlation coefficient between every sample to measure the degree of linear correlation between the two sets of data. Green indicates a negative correlation, and red indicates a positive correlation. Each group has two replicates and each experiment was repeated three times. (C) Distribution of peptides based on mass error. (D) Peptide length distribution of all peptides. (E) Distribution of peptides based on the number of ubiquitination sites. (F) Differentially expressed ubiquitin-modified sites and the corresponding proteins of different treatment groups (p < 0.05)