BMC Veterinary Research

Table 2 Comparison of C. psittaci LAMP assays using rapidly processed swab samples

From: Real-time fluorometric and end-point colorimetric isothermal assays for detection of equine pathogens C. psittaci and equine herpes virus 1: validation, comparison and application at the point of care

*C. psittaci* cLAMP^#	*C. psittaci* rtLAMP
		Positive	Negative	Total
	Positive	6	2	8
	Negative	0	26	26
	Total	6	28	34
	Kappa (0.95 CI; p(Kappa))	0.821 (0.584–1.06; 0.00)
	McNemar’s Chi square (p(Chi sq))	0.500 (0.479)
	Overall agreement ^b	94.12% (85.71% PA; 96.30% NA)
*C. psittaci* sgLAMP	*C. psittaci* rtLAMP
		Positive	Negative	Total
	Positive	6	1	7
	Negative	0	29	29
	Total	6	30	36
	Kappa (0.95 CI; p(Kappa))	0.906 (0.726–1.09; 0.00)
	McNemar’s Chi square (p(Chi sq))	0.000 (1.00)
	Overall agreement ^b	97.22% (92.31% PA; 98.31% NA)
*C. psittaci* sgLAMP	*C. psittaci* cLAMP^#
		Positive	Negative	Total
	Positive	6	1	7
	Negative	2	25	27
	Total	8	26	34
	Kappa (0.95 CI; p(Kappa))	0.744 (0.471–1.017; 0.00)
	McNemar’s Chi square (p(Chi sq))	0.000 (1.00)
	Overall agreement ^b	91.18% (80.00% PA; 94.34% NA)

^#: two samples were excluded from analyses as immediate discoloration of the mix (to yellow and/or orange) was observed upon addition the swab suspension; ^b: positive agreement (PA) and negative agreement (NA) are outlined in brackets

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ISSN: 1746-6148

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