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Table 1 C. psittaci and EHV-1 LAMP assays in comparison to reference diagnostic qPCR assays

From: Real-time fluorometric and end-point colorimetric isothermal assays for detection of equine pathogens C. psittaci and equine herpes virus 1: validation, comparison and application at the point of care

Reference C. psittaci qPCR
C. psittaci rtLAMP   Positive Negative Total
Positive 48 1 49
Negative 0 39 39
Total 48 40 88
Sensitivity a 100% (92.62–100%)
Specificity a 97.5% (86.84–99.94%)
Likelihood ratio + ve 40 (95% CI 5.77 to 277.06)
Likelihood ratio -ve 0
Overall agreement b 98.86% (98.97% PA; 98.73% NA)
EMAI Reference EHV-1 qPCR
EHV-1 rtLAMP   Positive Negative Total
Positive 20 0 20
Negative 3 12 15
Total 23 12 35
Sensitivity a 86.96% (66.41–97.22%)
Specificity a 100% (73.54–100%)
Likelihood ratio + ve N/A
Likelihood ratio -ve 0.130 (95% CI 0.05 to 0.37)
Overall agreement b 91.43% (93.02% PA; 88.89% NA)
IDEXX Reference EHV-1 qPCR
EHV-1 rtLAMP   Positive Negative Total
Positive 3 0 3
Negative 0 5 5
Total 3 5 8
Sensitivity a 100% (29.24–100%)
Specificity a 100% (47.82–100%)
Likelihood ratio + ve N/A
Likelihood ratio -ve 0.00
Overall agreement b 100%
  1. a: sensitivity and specificity are presented with specified Clopper-Pearson (exact) lower and upper 95% confidence limits, b: positive agreement (PA) and negative agreement (NA) are outlined in brackets. N/A: not applicable