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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Real-time fluorometric and end-point colorimetric isothermal assays for detection of equine pathogens C. psittaci and equine herpes virus 1: validation, comparison and application at the point of care

Fig. 1

Real-time and colorimetric C. psittaci and EHV-1 LAMP assays. The real-time fluorometer Genie III displays of (A) a C. psittaci and EHV-1 amplification curves within 30 min amplification time, and (B) High-resolution melts (HRM). Positive rtLAMP result is denoted with time to amplify (mm:ss) and HRM (°C), whilst negative result does not record either. End-point detection using C. psittaci (top row) and EHV-1 (bottom row) (C) cLAMP and (D) sgLAMP assays. cLAMP reaction resulting in yellow colour after amplification denote a positive result, and while pink colour represents a negative test result. sgLAMP reaction showing a bright yellow-green colour denote a positive test result and orange colour depict a negative result. The images were taken with iPhone SE

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