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Table 1 Reliability verification of RPA-LFD assay

From: Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus

   Serotypes of EHDV or PALV Total
   EHDV-1 EHDV-5 EHDV-6 EHDV-7 EHDV-10 BCV CHUV DAV
Virus strains Number of isolated viruses 11 9 12 4 3 7 17 5 68
RPA-LFD Positive 11 9 12 4 3 7 17 5 68
Negative 0 0 0 0 0 0 0 0 0
Coincidence rate 39/39 × 100 %=100 % 29/29 × 100 %=100 % 68/68 × 100 %=100 %
Blood samples CT values of qRT-PCR 28.937.5 33.737.8 29.537.7 30.138.0 32.637.9 33.537.6 28.237.1 30.336.3  
RPA-LFD Positive 8 7 9 4 3 6 12 5 54
Negative 1 0 0 0 0 0 1 0 2
Coincidence rate 31/32 × 100 %=96.9 % 23/24 × 100 %=95.8 % 54/56 × 100 %=96.4 %