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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus

Fig. 5

Trace back results of PALV infection obtained from qRT-PCR and the RPA-LFD assay. Top: Results of RPA nfo reactions detected by LFD detectors; Middle: results of qRT-PCR expressed with CT values; bottom: results of RPA nfo reactions analyzed by agarose gel electrophoresis. Lane 1–12: cDNAs of blood samples collected from sentinel animal infected with PALV served as templates; lane 13: negative control; lane 14: 100 bp DNA marker

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