Fig. 4

Trace back results of EHDV infection obtained from qRT-PCR and the RPA-LFD assay. Top: Results of RPA nfo reactions detected by LFD detectors; middle: results of qRT-PCR expressed with CT values; bottom: results of RPA nfo reactions analyzed by agarose gel electrophoresis. Lane 1–12: cDNAs of blood samples collected from sentinel animal infected with EHDV served as templates; lane 13: negative control; lane 14: 100 bp DNA marker