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Table 2 Summary of detection of the positive controls in the corresponding dilution for co-infections of six vector-borne pathogens. Numbers in the first bracket represent the number of times it was detected out of the number of times it was tested. Each sample was amplified for three variable regions of the 16S rRNA gene; V1-V2, V3-V4, and V4-V5

From: Data analysis workflow for the detection of canine vector-borne pathogens using 16 S rRNA Next-Generation Sequencing

Double Infection
A. phagocytophilum plus E. canis
Vector-borne pathogen 10 6 :10 6 10 5 :10 6 10 4 :10 6 10 3 :10 6 10 2 :10 6
A. phagocytophilum Yes(4/4) Yes(4/4) Yes(4/4) Yes(2/4) No(0/4)
E. canis Yes(4/4) Yes(4/4) Yes(4/4) Yes(4/4) Yes(4/4)
A. phagocytophilum plus B. henselae
Vector-borne pathogen 10 4 :10 4 10 3 :10 4 10 2 :10 4   
A. phagocytophilum Yes(4/4) Yes(4/4) Yes(4/4)   
B. henselae Yes(4/4) Yes(4/4) Yes(4/4)   
Triple infection
A. phagocytophilum plus E. canis plus B. vinsonii subsp. berkhoffii
Vector-borne pathogen 10 6 :10 6 :10 6 10 5 :10 6 :10 5 10 4 :10 6 :10 4 10 3 :10 6 :10 3 10 2 :10 6 :10 2
A. phagocytophilum Yes (6/6) Yes (6/6) Yes (6/6) Yes (3/6) No (0/6)
E. canis Yes (6/6) Yes (6/6) Yes (6/6) Yes (6/6) Yes (6/6)
B. vinsonii subsp. berkhoffii Yes (3/6) Yes (3/6) Yes (3/6) Yes (1/6) Yes (1/6)
E. canis plus B. vinsonii subsp. berkhoffii plus M. haemocanis
Vector-borne pathogen 10 4 :10 4 :10 4 10 4 :10 3 :10 4 10 4 :10 2 :10 4   
E. canis Yes (4/4) Yes (4/4) Yes (4/4)   
B. vinsonii subsp. berkhoffii Yes (4/4) Yes (4/4) Yes (1/4)   
M. haemocanis Yes (4/4) Yes (4/4) Yes (4/4)