Table 2 Summary of detection of the positive controls in the corresponding dilution for co-infections of six vector-borne pathogens. Numbers in the first bracket represent the number of times it was detected out of the number of times it was tested. Each sample was amplified for three variable regions of the 16S rRNA gene; V1-V2, V3-V4, and V4-V5
Double Infection | |||||
A. phagocytophilum plus E. canis | |||||
Vector-borne pathogen | 10 6 :10 6 | 10 5 :10 6 | 10 4 :10 6 | 10 3 :10 6 | 10 2 :10 6 |
A. phagocytophilum | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(2/4) | No(0/4) |
E. canis | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(4/4) |
A. phagocytophilum plus B. henselae | |||||
Vector-borne pathogen | 10 4 :10 4 | 10 3 :10 4 | 10 2 :10 4 | ||
A. phagocytophilum | Yes(4/4) | Yes(4/4) | Yes(4/4) | ||
B. henselae | Yes(4/4) | Yes(4/4) | Yes(4/4) | ||
Triple infection | |||||
A. phagocytophilum plus E. canis plus B. vinsonii subsp. berkhoffii | |||||
Vector-borne pathogen | 10 6 :10 6 :10 6 | 10 5 :10 6 :10 5 | 10 4 :10 6 :10 4 | 10 3 :10 6 :10 3 | 10 2 :10 6 :10 2 |
A. phagocytophilum | Yes (6/6) | Yes (6/6) | Yes (6/6) | Yes (3/6) | No (0/6) |
E. canis | Yes (6/6) | Yes (6/6) | Yes (6/6) | Yes (6/6) | Yes (6/6) |
B. vinsonii subsp. berkhoffii | Yes (3/6) | Yes (3/6) | Yes (3/6) | Yes (1/6) | Yes (1/6) |
E. canis plus B. vinsonii subsp. berkhoffii plus M. haemocanis | |||||
Vector-borne pathogen | 10 4 :10 4 :10 4 | 10 4 :10 3 :10 4 | 10 4 :10 2 :10 4 | ||
E. canis | Yes (4/4) | Yes (4/4) | Yes (4/4) | ||
B. vinsonii subsp. berkhoffii | Yes (4/4) | Yes (4/4) | Yes (1/4) | ||
M. haemocanis | Yes (4/4) | Yes (4/4) | Yes (4/4) | ||