Table 1 Summary of detection of the positive controls in the corresponding dilution for single-infection of six vector-borne pathogens. Numbers in the first bracket represent the number of times it was detected out of the number of times it was tested. Each sample was amplified for three variable regions of the 16S rRNA gene; V1-V2, V3-V4, and V4-V5
Single Infection | ||||||
|---|---|---|---|---|---|---|
Vector-borne pathogen | 10 6 | 10 5 | 10 4 | 10 3 | 10 2 | 10 1 |
A. phagocytophilum | Yes(2/4) | Yes(2/4) | Yes(2/4) | Yes(2/4) | Yes(1/4) | Yes(1/4) |
B. henselae | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(1/4) | No(0/4) |
B. vinsonii subsp. berkhoffii | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(3/4) | Yes(2/4) | No(0/4) |
E. canis | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(3/4) | Yes(2/4) |
E. chaffeensis | Yes(4/4) | Yes(4/4) | Yes(8/8) | Yes(8/8) | Yes(8/8) | Yes(1/4) |
M. haemocanis | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(4/4) | Yes(2/4) | No(0/4) |