Table 2 Sequence of genes and primers used for relative quantification by real-time PCR (qPCR) in hen’s uterine and liver tissues
Name of Target gene | Nucleotide sequence of primers (5′ → 3′) | Fragment Size (bp) | Reference (s) |
|---|---|---|---|
Oviduct genes | |||
 Ovocalyxin-32 (OCX32) | F: GGACAGCACTGCACTACATCAA | 514 | [38] |
R: GGAATTTCGTGGAGCAAGACAA | Â | Â | |
 Ovocalyxin-36 (OCX-36) | F: TTGGAATGGTCGTCTTCTGTGG | 121 | [39] |
R: CGGTCTGAATGATGGCATCG | Â | Â | |
 Ovocleidin-17 (OC-17) | F: CGTTCTGCCGCCGTTGGG | 96 | [40] |
R: CCCGCGACGCGTTGAGGA | Â | Â | |
 Ovocleidin-116 (OC-116) | F: AAGAGCCAACATCCAAGTGGGTGAGAAT | 424 | [41] |
R: CAGTGACCACATGGCTCCCTTTCCT | Â | Â | |
Hepatic selenoproteins | |||
 Glutathione peroxidase1 | F: GCGACTTCCTGCAGCTCAACGA | 99 | |
R: CGTTCTCCTGGTGCCCGAAT | Â | ||
 Glutathione peroxidase4 | F: CGGTGAATTACACTCAGCTCGT | 123 | |
R: CTTTGATCTGCGCGTCGTCC | Â | ||
 Iodothyronine deiodinase1 | F: AAGCTGCACCTGACCTTCATT | 138 | |
R: TTGTTTCTGAAGGCCCATCCA | Â | ||
 Iodothyronine deiodinase4 | F: CAGTGTAATCCACATAGCCA | 137 | |
R: CTGAGCCAAAATTAACCACC | Â | ||
 Selenoprotein W1 | F: CTCCGCGTCACCGTGCTCT | 155 | |
R: CTGCCCACCGTCACCTCGAAC | Â | ||
 Thioredoxin reductase1. | F: ACTGGATGACTATGACCGAA | 103 | |
R: TATGCATTCTCATACGTGAC | Â | ||
Housekeeping | |||
 Glyceraldehyde-3-phosphate dehydrogenase | F: AATGAGAGGTTCAGGTGCCC | 150 | |
R: ACCAGACAGCACTGTGTTGG | Â | ||
 β-actin8 | F: ACACACGGACACTTCAAGGG | 128 | |
R: TACTCAGCACCTGCATCTGC | Â | ||
 TATA-Box Binding Protein | F: TAGCCCGATGATGCCGTAT | 147 | |
R: GTTCCCTGTGTCGCTTGC | Â | Â | |