Effect of organic and inorganic dietary selenium supplementation on gene expression in oviduct tissues and Selenoproteins gene expression in Lohman Brown-classic laying hens

Table 1 Ingredient composition and analyzed nutrient levels of the basal diet (on dry matter basis)

Ingredients	Con	Na₂SeO₃	Se-Yeast	ADS18
Corn	44.00	44.00	44.00	44.00
Soybean meal 48%	29.00	29.00	29.00	29.00
Wheat pollard	11.00	11.00	11.00	11.00
CPO	3.50	3.50	3.50	3.50
L-Lysine	0.10	0.10	0.10	0.10
DL-methionine	0.25	0.25	0.25	0.25
Dicalcium phosphate (18%)	2.00	2.00	2.00	2.00
Calcium carbonate	7.70	7.70	7.70	7.70
Choline chloride	0.10	0.10	0.10	0.10
Salt	0.35	0.35	0.35	0.35
Mineral mix^a	0.60	0.597	0.597	0.597
Vitamin mix^b	0.60	0.60	0.60	0.60
Antioxidant^c	0.40	0.40	0.40	0.40
Toxin binder^d	0.40	0.40	0.40	0.40
Sodium selenite	0.00	0.003	0.00	0.00
Se-yeast	0.00	0.00	0.003	0.00
ADS18-Bacteria	0.00	0.00	0.00	0.003
Total	100	100	100	100
Analyzed composition
Metabolizable energy Kcal/Kg	2761.24	2761.24	2761.24	2761.24
Crude protein (%)	17.66	17.66	17.66	17.66
Crude fat (%)	5.3	5.3	5.3	5.3
Fibre (%)	3.98	3.98	3.98	3.98
Calcium (%)	3.65	3.65	3.65	3.65
Total Phosphorus (%)	0.88	0.88	0.88	0.88
Av. Phosphorus for poultry (%)	0.48	0.48	0.48	0.48
Analysed Se (mg/kg)^e	0.03 ± 0.01	0.31 ± 0.02	0.32 ± 0.01	0.33 ± 0.02

^aMineral premix provided (per kg of diet): Iron 120 mg, Manganese 150 mg, Copper 15 mg, Zinc 120 mg, Iodine 1.5 mg, and Cobalt 0.4 mg.^bVitamin premix supplied (per kg of diet): Vitamin A (retinyl acetate) 10.32 mg, Cholecalciferol 0.250 mg, Vitamin E (DL-tocopherol acetate) 90 mg, Vitamin K 6 mg, Cobalamin 0.07 mg, Thiamine 7 mg, Riboflavin 22 mg, Folic acid 3 mg, Biotin 0.04 mg, Pantothenic acid 35 mg, Niacin 120 mg and Pyridoxine 12 mg. ^cAntioxidant contains butylated hydroxyanisole (BHA). ^dToxin binder contains natural hydrated sodium calcium aluminum silicates to reduce the exposure of feed to mycotoxins. Feed live International Software (Nonthaburi, Thailand) was used to formulate the diets. ^eThe Se content measured using ICP.MS.

ISSN: 1746-6148