Fig. 3
Experimental design to study replication kinetics, clinical signs and case fatality rates in GI.1d/00–21-infected rabbits. Twenty-four rabbits (visualised in green or in red) were inoculated with a very high infective dose (around 1011 gRNA copies/rabbit) and two rabbits were euthanised at 16, 20, 24, 39 and 45 h post-infection (hpi), respectively. The assay was ended at 8 days post-infection (dpi). Rabbit in green: euthanised rabbit without RHD sign, rabbit in red: rabbit that died of RHD or was euthanised after reaching humane endpoints. Rabbit in light blue: uninfected control rabbit. * Blood and liver samples were collected from euthanised rabbits and only liver samples from animals found dead. RT-qPCR-positive samples (Table 1): L: liver, D: duodenum, RS: rectal swab, All: all the collected samples. +: detection of viral genome by RT-qPCR in the liver; −: absence of detection of viral genome by RT-qPCR in the liver; n.c.: sample not collected