Fig. 4
From: Impaired cerebrospinal fluid transport due to idiopathic subdural hematoma in pig: an unusual case
Microscopic investigation shows astrocyte dysfunction. (A-B) Representative images of hematoxylin and eosin staining of brain cortical surface and pial vessels from a control and an SDH pig, respectively. Scale bars = 2 mm. (C-D) Representative images of GFAP immunostaining (orange, astrocytes) of a control and an SDH pig, respectively, with BSA-647 tracer (magenta). Scale bar = 50 μm. (E) Quantification of the mean GFAP intensity in control and SDH pigs, n = 4 cortical regions analyzed in N = 1 animal per group. (F-I) Representative images of AQP4 immunostaining (orange, astrocyte water channel) and lectin (green, blood vessels) staining, with BSA-647 tracer (magenta) of a control (F-G) and an SDH pig (H-I). Scale bars (F, H) = 50 μm. Scale bars (G, I) = 10 μm. (J) Quantification of AQP4 polarization in a control and an SDH pig, n = 7 vessels analyzed per animal in N = 1 animal per group. AQP4, aquaporin-4; BSA-Alexa647, bovine serum albumin-AlexaFluor647; GFAP, glial-fibrillary acidic protein; SDH, subdural hematoma. Data represented as mean ± standard deviation (SD)