Fig. 3

Virus load of heart, liver, spleen, lung, kidney and brain in each group (n = 4). The mice were randomly dissected at 3, 4, and 5 dpi to collect these tissues. After homogenization with liquid nitrogen, the tissue samples (25 mg per sample) were used for DNA extraction. The virus gene copies were detected by FQ-PCR. The KM-H, KM-M and KM-L represent the PRV-infected groups which were treated with kaempferol at doses of 240, 160 and 80 mg/kg• body weight, respectively. ACV, the acyclovir-treated group (160 mg/kg• body weight). Symbols “*, ** and ***” represent p < 0.05, p < 0.01 and p < 0.001, respectively, between the infected-untreated control and the treated groups. Symbols “ξ, ξξ and ξξξ” represent p < 0.05, p < 0.01 and p < 0.001, respectively, between the acyclovir group and kaempferol groups