Angelica polysaccharide attenuates LPS-induced inflammation response of primary dairy cow claw dermal cells via NF-κB and MAPK signaling pathways

Table 1 Primer sequences used for amplification of qPCR

Accession No.	Genes^a	Sense	Antisense	Product size(bp)
NM_174198.6	TLR4	AGCTTCAACCGTATCATGGCCTCT	ACTAAGCACTGGCATGTCCTCCAT	166
NM_001014382.2	MyD88	AAGTACAAGCCAATGAAGAAAGAG	GAGGCGAGTCCAGAACCAG	102
NM_174006.2	CCL2	CGCTCAGCCAGATGCAATTA	GACCCATTTCTGCTTGGGGT	184
NM_174263.2	CCL20	TTGATGTCAGTGCTATTGCT	ACCCACTTCTTCTTTGGATC	209
NM_174299.3	CXCL2	ACCGAAGTCATAGCCACTCTC	TCCAGATGGCCTTAGGAGGT	218
NM_173925.2	CXCL8	AAACACATTCCACACCTTTC	TCTTCACAAATACCTGCACA	171
NM_001046551	CXCL10	CTCGAACACGGAAAGAGGCA	TCCACGGACAATTAGGGCTT	117
NM_001034034.2	GAPDH	CACCCTCAAGATTGTCAGCA	GGTCATAAGTCCCTCCACGA	103

^aTLR4, toll-like receptor 4, MyD88, myeloid differentiation factor 88, GAPDH, Glyceraldehyde 3-phosphate dehydrogenase

ISSN: 1746-6148