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Table 5 The effect of protease supplementation in different feeding regimens on the blood biochemical parameters of broiler chickens

From: Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens

Item Feeding regimens Protease level Feeding regimens × Protease level P-value SEM
FR1 FR2 FR3 − VE + VE FR1 − VE FR1 + VE FR2 − VE FR2 + VE FR3 − VE FR3 + VE FR Protease Interaction
TP (g/dL) 7.98 7.517 8.40 7.47 8.46 7.72 8.24 7.40 7.63 7.29 9.51 0.59 0.17 0.48 0.33
Albumin (g/dL) 4.08 4.20 4.35 4.23 4.18 4.1 4.06 4.11 4.29 4.50 4.20 0.08 0.55 0.13 0.05
Globulin (g/dL) 3.69 3.31 4.05 3.17 4.19 3.44 3.94 3.28 3.34 2.79 5.30 0.65 0.13 0.29 0.32
A/G ratio (%) 1.25 1.28 1.39 1.49 1.12 1.38 1.123 1.29 1.28 1.82 0.96 0.87 0.11 0.32 0.11
ALP(U/L) 60.22ab 48.48b 74.23a 59.59 62.36 57.49 62.96 50.23 46.73 71.07 77.39 0.04 0.71 0.83 4.01
C3 (mg/dL) 109.16 99.98 117.63 101.17 116.68 110 108.33 86.51 113.46 107 128.27 0.27 0.08 0.35 4.60
IgM (mg/dL) 94.73b 74.36b 142.56a 87.80b 119.97a 94.77b 94.69b 73.04b 75.69b 95.59b 189.52a 0.01 0.04 0.04 10.78
  1. FR1: recommended protein corn-SBM diet; FR2: low-protein SBM diet; FR3: low-protein SBM/DDGS-SFM diet; FR1 − VE: recommended protein SBM diet without protease supplementation, FR1 + VE: recommended protein SBM diet + protease supplementation, FR2 − VE: low-protein SBM diet without protease supplementation, FR2 + VE: low-protein SBM diet + protease supplementation, FR3 − VE: low-protein SBM/DDGS-SFM diet without protease supplementation, FR3 + VE: low-protein SBM/DDGS-SFM diet + protease supplementation; TP: total protein; A/G ratio: albumin/globulin ratio; ALP: alkaline phosphatase; C3: complement 3
  2. a,bMeans within the same row carrying different superscripts are significantly different at (P < 0.05)