Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens

Table 5 The effect of protease supplementation in different feeding regimens on the blood biochemical parameters of broiler chickens

Item	Feeding regimens			Protease level		Feeding regimens × Protease level						P-value			SEM
Item	FR1	FR2	FR3	− VE	+ VE	FR1 − VE	FR1 + VE	FR2 − VE	FR2 + VE	FR3 − VE	FR3 + VE	FR	Protease	Interaction	SEM
TP (g/dL)	7.98	7.517	8.40	7.47	8.46	7.72	8.24	7.40	7.63	7.29	9.51	0.59	0.17	0.48	0.33
Albumin (g/dL)	4.08	4.20	4.35	4.23	4.18	4.1	4.06	4.11	4.29	4.50	4.20	0.08	0.55	0.13	0.05
Globulin (g/dL)	3.69	3.31	4.05	3.17	4.19	3.44	3.94	3.28	3.34	2.79	5.30	0.65	0.13	0.29	0.32
A/G ratio (%)	1.25	1.28	1.39	1.49	1.12	1.38	1.123	1.29	1.28	1.82	0.96	0.87	0.11	0.32	0.11
ALP(U/L)	60.22ab	48.48b	74.23a	59.59	62.36	57.49	62.96	50.23	46.73	71.07	77.39	0.04	0.71	0.83	4.01
C3 (mg/dL)	109.16	99.98	117.63	101.17	116.68	110	108.33	86.51	113.46	107	128.27	0.27	0.08	0.35	4.60
IgM (mg/dL)	94.73^b	74.36^b	142.56^a	87.80^b	119.97^a	94.77^b	94.69^b	73.04^b	75.69^b	95.59^b	189.52^a	0.01	0.04	0.04	10.78

FR1: recommended protein corn-SBM diet; FR2: low-protein SBM diet; FR3: low-protein SBM/DDGS-SFM diet; FR1 − VE: recommended protein SBM diet without protease supplementation, FR1 + VE: recommended protein SBM diet + protease supplementation, FR2 − VE: low-protein SBM diet without protease supplementation, FR2 + VE: low-protein SBM diet + protease supplementation, FR3 − VE: low-protein SBM/DDGS-SFM diet without protease supplementation, FR3 + VE: low-protein SBM/DDGS-SFM diet + protease supplementation; TP: total protein; A/G ratio: albumin/globulin ratio; ALP: alkaline phosphatase; C3: complement 3
^a,bMeans within the same row carrying different superscripts are significantly different at (P < 0.05)

ISSN: 1746-6148