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Table 4 The effect of different feeding regimens, protease supplementation, or their interaction on the intestinal histomorphometric measures of broiler chickens

From: Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens

Item Feeding regimens Protease level Feeding regimens × Protease level P-value SEM
FR1 FR2 FR3 − VE + VE FR1 − VE FR1 + VE FR2 − VE FR2 + VE FR3 − VE FR3 + VE FR Protease Interaction
Duodenum
 VH (µm) 817.26a 766.05a 449.56b 711.71 643.53 821.71 812.82 821.71 710.38 491.71 407.40 0.00 0.23 0.74 47.82
 VW (µm) 98.91 121.30 82.02 98.97 102.52 104.33 93.50 104.33 138.28 88.25 75.78 0.32 0.87 0.59 10.00
 CD (µm) 251.83 247.74 284.26 229.95b 292.61a 241.66b 262.00b 241.66b 253.83b 206.52b 362.01a 0.39 0.02 0.04 15.73
 VH/CD 3.32a 2.04b 2.25ab 2.82 2.25 3.49a 3.15a 2.99ab 1.10b 1.99ab 2.51ab 0.02 0.12 0.03 0.24
 GCC 65.75 75.33 79.66 64.66 82.50 61.00 70.50 61.00 89.66 72.00 87.33 0.72 0.22 0.85 6.42
Jejunum
 VH (µm) 753.01a 666.94ab 360.09b 603.60 583.09 777.53 728.47 777.53 556.36 255.75 464.44 0.03 0.85 0.29 66.20
 VW (µm) 102.03a 99.88a 59.04b 89.314 84.66 104.92 99.14 104.92 94.85 58.09 60.01 0.009 0.66 0.88 6.67
 CD (µm) 349.40 305.51 340.41 319.81 343.73 352.22 346.59 352.22 258.79 254.99 425.82 0.90 0.78 0.44 38.33
 VH/CD 2.18ab 1.43b 3.12a 2.66a 1.83b 2.22 2.13 2.21 0.65 3.55 2.69 0.002 0.01 0.17 0.25
 GCC 58.75 65.16 77.00 78.00a 55.94b 63.00b 54.50b 63.00b 67.33b 108.00a 46.00b 0.30 0.03 0.02 6.26
Ileum
 VH (µm) 442.32 407.3 251.67 340.35 393.91 390.67 493.97 390.67 424.12 239.70 263.65 0.08 0.44 0.87 34.78
 VW (µm) 111.82a 105.67a 59.83b 89.68 95.21 104.39 119.25 104.39 106.96 60.24 59.43 0.01 0.67 0.87 7.80
 CD (µm) 110.23b 112.131b 205.48a 134.21 151.01 113.14 107.31 113.14 111.11 176.35 234.62 0.03 0.59 0.63 17.35
 VH/CD 4.53 1.48 3.13 2.74 3.35 3.91 5.14 1.91 1.05 2.40 3.85 0.06 0.51 0.52 0.49
 GCC 107.08 109.16 93.66 98.55 108.05 101.66 112.50 101.66 116.66 92.33 95.00 0.70 0.57 0.95 7.14
  1. FR1: recommended protein corn-SBM diet, FR2: low-protein SBM diet, FR3: low-protein SBM/DDGS-SFM diet; FR1 − VE: recommended protein SBM diet without protease supplementation, FR1 + VE: recommended protein SBM diet + protease supplementation, FR2 − VE: low-protein SBM diet without protease supplementation, FR2 + VE: low-protein SBM diet + protease supplementation, FR3 − VE: low-protein SBM/DDGS-SFM diet without protease supplementation, FR3 + VE: low-protein SBM/DDGS-SFM diet + protease supplementation; VH: villous height; VW: villous width; CD: crypt depth; GCC: goblet cell count; VH/CD: villous height to crypt depth
  2. a,bMeans within the same row carrying different superscripts are significantly different at (P < 0.05)