Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens

Amer, Shimaa A.; Beheiry, Rasha R.; Abdel Fattah, Doaa M.; Roushdy, Elshimaa M.; Hassan, Fardos A. M.; Ismail, Tamer Ahmed; Zaitoun, Noha M. A.; Abo-Elmaaty, Azza M. A.; Metwally, Abdallah E.

doi:10.1186/s12917-021-02946-2

Table 4 The effect of different feeding regimens, protease supplementation, or their interaction on the intestinal histomorphometric measures of broiler chickens

From: Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens

Item	Feeding regimens			Protease level		Feeding regimens × Protease level						P-value			SEM
Item	FR1	FR2	FR3	− VE	+ VE	FR1 − VE	FR1 + VE	FR2 − VE	FR2 + VE	FR3 − VE	FR3 + VE	FR	Protease	Interaction	SEM
Duodenum
VH (µm)	817.26^a	766.05^a	449.56^b	711.71	643.53	821.71	812.82	821.71	710.38	491.71	407.40	0.00	0.23	0.74	47.82
VW (µm)	98.91	121.30	82.02	98.97	102.52	104.33	93.50	104.33	138.28	88.25	75.78	0.32	0.87	0.59	10.00
CD (µm)	251.83	247.74	284.26	229.95^b	292.61^a	241.66^b	262.00^b	241.66^b	253.83^b	206.52^b	362.01^a	0.39	0.02	0.04	15.73
VH/CD	3.32^a	2.04^b	2.25^ab	2.82	2.25	3.49^a	3.15^a	2.99^ab	1.10^b	1.99^ab	2.51^ab	0.02	0.12	0.03	0.24
GCC	65.75	75.33	79.66	64.66	82.50	61.00	70.50	61.00	89.66	72.00	87.33	0.72	0.22	0.85	6.42
Jejunum
VH (µm)	753.01^a	666.94^ab	360.09^b	603.60	583.09	777.53	728.47	777.53	556.36	255.75	464.44	0.03	0.85	0.29	66.20
VW (µm)	102.03^a	99.88^a	59.04^b	89.314	84.66	104.92	99.14	104.92	94.85	58.09	60.01	0.009	0.66	0.88	6.67
CD (µm)	349.40	305.51	340.41	319.81	343.73	352.22	346.59	352.22	258.79	254.99	425.82	0.90	0.78	0.44	38.33
VH/CD	2.18^ab	1.43^b	3.12^a	2.66a	1.83b	2.22	2.13	2.21	0.65	3.55	2.69	0.002	0.01	0.17	0.25
GCC	58.75	65.16	77.00	78.00^a	55.94^b	63.00^b	54.50^b	63.00^b	67.33^b	108.00^a	46.00^b	0.30	0.03	0.02	6.26
Ileum
VH (µm)	442.32	407.3	251.67	340.35	393.91	390.67	493.97	390.67	424.12	239.70	263.65	0.08	0.44	0.87	34.78
VW (µm)	111.82^a	105.67^a	59.83^b	89.68	95.21	104.39	119.25	104.39	106.96	60.24	59.43	0.01	0.67	0.87	7.80
CD (µm)	110.23^b	112.131^b	205.48^a	134.21	151.01	113.14	107.31	113.14	111.11	176.35	234.62	0.03	0.59	0.63	17.35
VH/CD	4.53	1.48	3.13	2.74	3.35	3.91	5.14	1.91	1.05	2.40	3.85	0.06	0.51	0.52	0.49
GCC	107.08	109.16	93.66	98.55	108.05	101.66	112.50	101.66	116.66	92.33	95.00	0.70	0.57	0.95	7.14

FR1: recommended protein corn-SBM diet, FR2: low-protein SBM diet, FR3: low-protein SBM/DDGS-SFM diet; FR1 − VE: recommended protein SBM diet without protease supplementation, FR1 + VE: recommended protein SBM diet + protease supplementation, FR2 − VE: low-protein SBM diet without protease supplementation, FR2 + VE: low-protein SBM diet + protease supplementation, FR3 − VE: low-protein SBM/DDGS-SFM diet without protease supplementation, FR3 + VE: low-protein SBM/DDGS-SFM diet + protease supplementation; VH: villous height; VW: villous width; CD: crypt depth; GCC: goblet cell count; VH/CD: villous height to crypt depth
^a,bMeans within the same row carrying different superscripts are significantly different at (P < 0.05)

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ISSN: 1746-6148

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