Fig. 5

Impact of bacterial stimulation on the composition of camel monocytes. Camel leukocytes were separated from unstimulated and stimulated blood and were labeled with monoclonal antibodies to CD14 and MHCII molecules. Labeled cells were analyzed by flow cytometry. (A) Gating strategy of camel monocyte subsets. After setting agate on MNC, Camel monocytes were identified based on their forward (FSC) and side scatter (SSC) properties. In a separate FSC-A/FSC-H dot plot, a gate was made on single cells (exclusion of duplets). (B) Monocyte subsets Mo-I, Mo-II, and Mo-III were identified according to their CD14 and MHCII expression density (Mo-I: CD14^highMHCII^low, Mo-II: CD14^highMHCII^high, Mo-III: CD14^lowMHCII^high). (C) The percentage of each monocyte subset was calculated and presented for unstimulated and stimulated blood. Differences between groups were considered significant (*) if P < 0.05