Fig. 2
From: Bacterial species-specific modulatory effects on phenotype and function of camel blood leukocytes
Flow cytometric analysis of ROS production in camel blood leukocytes. Camel leukocytes were separated from unstimulated and stimulated blood and separated cells were labeled with the ROS-sensitive dye DHR-123 and labeled cells were analyzed by flow cytometry A) In a SSC-A/FSC-A dot plot, a gate was seton leukocytes and duplets were excluded in a FSC-A/FSC-H dot plot. Camel granulocytes (G) and mononuclear cells (MNC) were gated according to their forward and side scatter characteristics. In the MNC gate, monocytes (M) and lymphocytes (L) were identified based on their different forward and side scatter characteristics. In a SSC-A/FL-1 dot plot, the percentage of DHR-positive cells and the mean green fluorescence intensity for each cell population were calculated