Skip to main content
Fig. 1 | BMC Veterinary Research

Fig. 1

From: Bacterial species-specific modulatory effects on phenotype and function of camel blood leukocytes

Fig. 1

Gating strategy for the identification of the main leukocyte populations in camel blood and the analysis of their viability. (A) Separated camel leukocytes were labeled with propidium iodide and analyzed by flow cytometry. In a SSC/FSC dot plot, camel granulocytes (G) and mononuclear cells (MNC) were gated according to their forward and side scatter characteristics. In the MNC gate, monocytes (M) and lymphocytes (L) were identified based on their different forward and side scatter characteristics. In a separate FSC-A/FSC-H dot plot for each cell population, a gate was made on single cells (exclusion of duplets). In a SSC-A/FL-3 dot plot, viable cells were identified as PI-negative cells. (B) For unstimulated cells, cells stimulated with E. coli, S. aureus, and S. agalactiae, the percentages of viable cells among granulocytes, monocytes, and lymphocytes were calculated and data were presented as mean ± SEM

Back to article page