Fig. 1

Phylogenetic tree of HEV nucleotide sequences based on partial ORF1 (242 nt). The maximum likelihood phylogenetic tree was built with GTR + G + I substitution model and a bootstrap resampling process (1000 replications) was used to assess node support. Bootstrap values > 70 are indicated at their respective nodes. The tree included 66 HEV sequences: i) 28 Slovak HEV sequences are indicated by a black circle ii) 38 HEV sequences were selected from NCBI GenBank database, HEV-3 reference subtypes according Smith et al. [13] are indicated by a black triangle. Highly diverse sequences outside known subtypes are marked with a question mark. The HEV-4 strain was used as an outgroup. All sequences are denoted by name sequences, ISO code country of origin and NCBI GenBank accession number in brackets. Human HEV sequences are marked with an asterix. The scale bar indicates nucleotide substitutions per site