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Table 7 Chemical composition of Chlorella vulgaris and experimental diets

From: Impact of dietary Chlorella vulgaris and carbohydrate-active enzymes incorporation on plasma metabolites and liver lipid composition of broilers

  Microalga Experimental Diets
Item C. vulgaris Control CH CHR CHM
Energy, kcal ME/kg as fed basis 4586 4614 4627 4650 4615
Proximate composition, % as fed basis
Dry matter 93.1 89.0 89.6 89.3 86.4
Crude protein 42.8 19.9 20.4 19.8 19.1
Crude fat 8.73 6.59 7.56 7.63 7.41
Ash 11.8 5.60 6.08 6.21 6.13
Estimated available limiting amino acid composition, % as fed basis
Arginine 3.89 1.42 1.08 1.08 1.08
Histidine 0.65 0.55 0.43 0.43 0.43
Isoleucine 1.26 1.04 0.78 0.78 0.78
Leucine 2.45 1.81 1.44 1.44 1.44
Lysine 2.63 1.13 1.11 1.11 1.11
Methionine 0.45 0.60 0.61 0.61 0.61
Phenylalanine 1.49 1.13 0.87 0.87 0.87
Threonine 2.32 0.79 0.60 0.60 0.60
Tryptophan 0.47 0.29 0.22 0.22 0.22
Valine 3.52 1.12 0.86 0.86 0.86
Fatty acid profile, % total fatty acids
C14:0 1.10 0.10 0.14 0.14 0.19
C16:0 17.2 12.4 12.6 12.6 13.2
C16:1c9 3.90 0.09 0.95 0.98 1.15
C18:0 3.00 2.77 2.83 2.81 2.99
C18:1c9 11.7 21.6 22.1 22.6 23.2
C18:1c11 0.00 1.35 1.63 1.58 1.81
C18:2n-6 11.2 50.5 47.3 48.0 46.5
C18:3n-3 10.1 5.24 5.47 5.58 5.62
C20:0 0.20 0.33 0.32 0.33 0.33
C20:1c11 0.10 0.22 0.25 0.23 0.27
Diterpene profile, µg/g      
α-Tocopherol 19.2 10.5 42.2 12.4 20.2
α-Tocotrienol n.d.+ 1.29 5.94 3.00 2.75
β-Tocopherol 0.34 0.44 0.98 0.52 0.66
γ-Tocopherol + β-tocotrienol 0.52 16.2 26.8 14.7 19.3
γ-Tocotrienol 0.56 2.50 7.60 3.92 3.36
δ-Tocopherol 0.36 2.00 4.44 2.79 2.90
Pigments, µg/g      
β-Carotene 198 n.d. 83.6 37.3 45.1
Chlorophyll a1 906 0.67 307 339 200
Chlorophyll b2 171 0.90 96.3 104 40.0
Total chlorophylls3 1077 1.57 404 444 240
Total carotenoids4 228 3.61 102 108 47.7
Total chlorophylls + Carotenoids5 1305 5.17 505 552 288
  1. The broilers were fed: (1) a corn-soybean based diet (Control); (2) the based diet plus 10 % C. vulgaris (CH); (3) diet 2 supplemented with 0.005 % Rovabio® Excel AP; and (4) diet 2 supplemented with 0.01 % of a pre-selected four-CAZyme mixture (CHM)
  2. DM - dry matter; ME - metabolized energy; n.d. - not detected
  3. + Co-eluted with α-tocopherol
  4. 1 Ca = 11.24 × A662 − 2.04 × A645
  5. 2 Cb = 0.13 × A645 − 4.19 × A662
  6. 3 Ca + b = 7.05 × A662 + 18.09 × A645
  7. 4 Cx + c = (1000 × A470 − 1.90 × Ca − 63.14 × Cb) /214
  8. 5 (Ca + b) + (Cx + c)