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Table 3 Effect of experimental diets on hepatic total lipids, total cholesterol and fatty acid (FA) composition of broilers

From: Impact of dietary Chlorella vulgaris and carbohydrate-active enzymes incorporation on plasma metabolites and liver lipid composition of broilers

Item Control CH CHR CHM SEM p-value
Total lipids, g/100 g 3.10 2.90 3.20 2.98 0.126 0.363
Total cholesterol, mg/g 1.09 1.17 1.18 1.06 0.073 0.614
FA composition, g/100 g FA
C10:0 0.01 0.00 0.01 0.00 0.002 0.192
C12:0 0.01 0.01 0.01 0.01 0.003 0.446
C14:0 0.22 0.17 0.22 0.20 0.025 0.395
C14:1c9 0.02 0.01 0.01 0.01 0.005 0.851
C15:0 0.05 0.05 0.05 0.05 0.005 0.988
DMA-C16:0 0.11 0.13 0.13 0.11 0.018 0.819
C16:0 19.6 17.6 18.6 18.6 0.920 0.533
C16:1c7 0.18a 0.28b 0.27b 0.30b 0.014 < 0.001
C16:1c9 0.69 0.46 0.48 0.62 0.135 0.580
C17:0 0.34 0.41 0.41 0.40 0.028 0.210
C17:1c9 0.03 0.04 0.04 0.04 0.005 0.108
DMA-C18:0 0.18 0.26 0.26 0.21 0.037 0.369
DMA-C18:1 0.02 0.01 0.02 0.02 0.004 0.848
C18:0 22.9 24.4 24.3 23.6 0.540 0.222
C18:1c9 12.9 10.1 10.5 12.2 1.628 0.562
C18:1c11 1.11 1.26 1.22 1.28 0.044 0.048
C18:2n-6 20.0 20.5 20.6 20.0 0.603 0.860
C18:3n-6 0.03 0.04 0.04 0.04 0.003 0.255
C18:2t9t12 0.14 0.14 0.13 0.15 0.011 0.794
C18:3n-3 0.46 0.56 0.57 0.58 0.035 0.099
C18:4n-3 0.01 0.02 0.02 0.02 0.002 0.100
C20:0 0.14 0.15 0.14 0.14 0.009 0.806
C20:1c11 0.29 0.24 0.24 0.25 0.021 0.282
C20:2n-6 1.09 1.21 1.13 1.04 0.085 0.564
C20:3n-6 1.13 1.04 1.03 1.11 0.095 0.843
C20:4n-6 11.7 13.1 12.1 11.5 1.034 0.695
C20:3n-3 0.08a 0.12b 0.12b 0.11ab 0.009 0.032
C20:5n-3 0.22 0.28 0.30 0.29 0.022 0.065
C22:0 0.07 0.07 0.07 0.07 0.005 0.973
C22:1n-9 0.01 0.01 0.01 0.01 0.002 0.589
C22:2n-6 0.015 0.012 0.016 0.012 0.002 0.316
C22:5n-3 1.24 1.18 1.07 1.02 0.111 0.481
C22:6n-3 1.95 3.02 2.79 2.63 0.324 0.130
Others 3.56 2.82 3.35 2.98 0.343 0.426
Partial sums of FA, g/100 g FA
SFA1 43.3 42.8 43.8 43.1 0.568 0.659
MUFA2 15.2 12.4 12.8 14.8 1.785 0.601
PUFA3 38.1 41.2 39.9 38.5 1.932 0.667
n-6 PUFA4 34.0 35.9 34.9 33.7 1.594 0.773
n-3 PUFA5 3.97 5.18 4.87 4.64 0.396 0.191
Ratios of FA
PUFA/SFA 0.88 0.97 0.92 0.90 0.053 0.672
n-6/n-3 8.79a 7.22b 7.38ab 7.57ab 0.382 0.025
  1. The broilers were fed: (1) a corn-soybean based diet (Control); (2) the based diet plus 10% C. vulgaris (CH); (3) diet 2 supplemented with 0.005% Rovabio® Excel AP; and (4) diet 2 supplemented with 0.01% of a pre-selected four-CAZyme mixture (CHM)
  2. SEM - standard error of the mean; FA - fatty acids; DMA - dimethylacetal; SFA - saturated fatty acids; MUFA - monounsaturated fatty acids; PUFA - polyunsaturated fatty acids
  3. 1 Sum (C10:0, C12:0, C14:0, C15:0, C16:0, C17:0, C18:0, C20:0, C22:0)
  4. 2 Sum (C14:1c9, C16:1c7, C16:1c9, C17:1c9, C17:1c10, C18:1c9, C18:1c11, C20:1c11, C22:1n-9)
  5. 3 Sum (C18:2n-6, C18:2t9t12, C18:3n-6, C18:3n-3, C18:4n-3, C20:2n-6, C20:3n-6, C20:4n-6, C20:3n-3, C20:5n-3, C22:5n-3, C22:6n-3)
  6. 4 Sum (C18:2n-6, C18:3n-6, C20:2n-6, C20:3n-6, C20:4n-6)
  7. 5 Sum (C18:3n-3, C18:4n-3, C20:3n-3, C20:5n-3, C22:5n-3, C22:6n-3)
  8. a.b Different superscripts within a row indicate a significant difference (p < 0.05)